Effect on invasion and migration of migration of mitofusion-2 overexpression to phosphatidylinositol transfer membrane-associated protein 3-positive hepatoma cell Line SMMC-7721

Abstract

Objective To investigate the effect on invasion and migration of mitofusion-2 (Mfn-2) overexpression to phosphatidylinositol transfer membrane-associated protein 3 (PITPNM3)-positive hepatoma cell Line SMMC-7721. Methods PITPNM3 positive liver cancer cell line was selected for experiment while PITPNM3 positive breast cancer cell line McF-7 was used as a positive control group. 20 μg/L CC-chemokine ligand 18 (CCL18) was used to establish a PITPNM3 positive hepatoma cell model with invasion and migration. PITPNM3 positive hepatoma cell were transfected Mfn-2 with an adenoviral vector after 36 h to detect the change of PITPNM3 expression and its effect on invasion and migration of hepatoma cells. Results Compared to other groups, PITPNM3 was highly expressing in hepatoma cell line SMMC-7721, the relative expression level of PITPNM3 is 3.4 in SMMC-7721, 1.6 in HepG2 and 4.5 in McF-7 (SMMC-7721 vs. HepG2, t=3.653, P 0.05); 20 μg/L CCL18 can effectively promote invasion and migration of hepatoma cell line SMMC-7721 with PITPNM3 high expression, by suppressing the expression of E-Cadherin and up-regulating the expressions of Snail and Vimentin; Overexpression of Mfn-2 leads to a significant decrease in PITPNM3 expression and suppression on invasion and migration in hepatoma cell line SMMC-7721, but has no effects on the mRNA level of PITPNM3. The relative expression level of PITPNM3 is 0.903 in experimental group and 1.128 in positive control group (experimental group vs. positive control group, P>0.05). Conclusion Mfn-2 can effectively down-regulate the expression of PITPNM3 and inhibit the invasion and migration of hepatoma cell line SMMC-7721. Key words: Mitofusin-2; Phosphatidylinositol transfer membrane-associated protein 3; Hepatocellular carcinoma; Invasion; Migration