Abstract
The present study explored the effect of long non-coding RNA-human ovarian cancer-specific transcript 2 (LncRNA-HOST2) on cell proliferation, migration, invasion and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721. HCC tissues and adjacent normal tissues from 162 HCC patients were collected. The HCC cell lines were assigned into the control group (regular culture), negative control (NC) group (transfected with siRNA) and experimental group (transfected with Lnc-HOST2 siRNA). Quantitative real-time PCR (qRT-PCR) was used to detect the expression of LncRNA-HOST2. Cell proliferation was detected by CCK-8 and colony-forming assays, cell apoptosis by flow cytometry and cell migration by Scratch test. Transwell assay was used to evaluate cell migration and invasion abilities. LncRNA-HOST2 expression in the HCC tissues increased 2–10 times than that in the adjacent normal tissues. Compared with the HL-7702 cell line, LncRNA-HOST2 expression in HepG2, SMMC-7721 and Huh7 cell lines was all up-regulated, but the SMMC-7721 cell had the highest Lnc-HOST2 expression. The LncRNA-HOST2 expression in the experimental group was down-regulated as compared with the control and NC groups. In comparison with the control and NC groups, cloned cells reduced, cell apoptosis increased, clone-forming ability weakened and inhibitory rate of colony formation increased in the experimental group. The cells migrating and penetrating into the transwell chamber were fewer in the experimental group than those in the control and NC groups. The experimental group exhibited slow wound healing and decreased cell migration area after 48 h. These findings indicate that LncRNA-HOST2 can promote cell proliferation, migration and invasion and inhibit cell apoptosis in human HCC cell line SMMC-7721.
Highlights
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide with its incidence rapidly increasing in the recent years [1]
Results showed that the expression of Long non-coding RNA (LncRNA)-Human ovarian cancer-specific transcript 2 (HOST2) in HCC tissues was up-regulated by approximately 2–10 times
When the LncRNA-HOST2 expression in HCC tissues was lower than two times of adjacent normal tissues, it was considered as low expression, and the LncRNA-HOST2 expression higher than two times was considered high expression
Summary
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide with its incidence rapidly increasing in the recent years [1]. It is estimated that 1 million people are expected to die from HCC annually since this disease is easy to be confused with cirrhosis, posing a great challenge for the early diagnosis and resulting in a sharp increase in HCC patients [2,3]. Many factors may result in HCC, among which infection of hepatitis A virus (HAV) or hepatitis B virus (HBV) tops [4]. Chemical and biological factors including heredity, signalling pathway disorder and protein dysfunction can lead to cell damage, which remains one of the important causes of HCC [5]. It is very important to understand tumour characteristics, liver function and physiological condition from various aspects for the diagnosis and treatment of HCC
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
