Abstract
Severe burn trauma triggers oxidative gastric mucosal injury. The purpose ofthis study was toinvestigate the antioxidant defence mechanisms and protective effect ofmelatonin inthe gastric mucosa after burn injury. Inorder toinvestigate the mechanisms involved inthe gastric antioxidant defence inarat burn model, quantitative real-time PCR and immunohistochemistry techniques were applied. Ananalysis ofglutathione peroxidase4 (GPx4), glutathione reductase (GR), and catalase (Cat) gene expression was performed along with the evaluation ofthe gastric Cu/Zn superoxide dismutase (Cu/Zn SOD) activity. Melatonin was applied immediately and 12h after 30% oftotal body surface area burns. The burn injury significantly increased the Gpx4 mRNA (P<0.0001) and Gsr mRNA (P<0.0001) expression. Italso had aslight positive effect onthe Cat mRNA expression and Cu/Zn SOD activity. Melatonin, inturn, markedly augmented the burn-induced Cu/Zn SOD (P<0.0001) activity, reversed the Gpx4 mRNA (P<0.0001) and Gsr mRNA (P<0.0001) expression, and inhibited the Cat mRNA level. Inconclusion, the present study suggests that aburn injury adaptively increases the Cu/Zn SOD activity and enhances the Gpx4 and Gsr gene expression inthe gastric mucosa. Melatonin effectively modulates the expression ofthe cellular antioxidant enzymes, and improves the antioxidant defence byaugmenting the Cu/Zn SOD activity.
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