Abstract
The present study was aimed to examine the effects of 3-week zinc and melatonin administration on testicular tissue injury and serum Inhibin-B levels caused by unilateral testicular torsion-detorsion in rats. The study was performed on 60 Wistar Albino-type adult male rats. The animals were allocated to 6 groups in equal numbers. 1. Control; 2. Sham; 3. Ischemia-reperfusion; 4. Zinc+ischemia-reperfusion; 5. Melatonin+ischemia-reperfusion; 6. Zinc+melatonin+ischemia-reperfusion. Zinc and melatonin were administered before ischemia-reperfusion at doses of 5 and 3mg/kg respectively, by intraperitoneal route for a period of 3weeks. Testicular torsion-detorsion procedures consisted of ischemia for 1h and then reperfusion for another hour of the left testis. Blood and testicular tissue samples were collected to analyze erythrocyte and tissue GSH and plasma and tissue MDA, Inhibin-B levels. The highest erythrocyte and testis GSH values were found in zinc, melatonin, and zinc+melatonin groups (p<0.001). Torsion-detorsion group has significantly lower erythrocyte GSH levels and higher plasma MDA values (p<0.001). Serum inhibin-B and spermatogenic activity levels in the torsion-detorsion group were also significantly lower than those in the other groups (p<0.001). However, zinc-, melatonin-, and melatonin+zinc-supplemented groups have higher inhibin-B and spermatogenetic activity (p<0.001). The results of the study show that zinc, melatonin, and melatonin+zinc administration partially restores the increased oxidative stress, as well as the reduced inhibin-B and spermatogenic activity levels in testes ischemia-reperfusion in rats. Suppressed inhibin-B levels in the testicular tissue may be a marker of oxidative stress.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call