Abstract
X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology. The recently available MHz repetition rate XFELs allow full data sets to be collected in shorter time and can also decrease sample consumption. However, the microsecond spacing of MHz XFEL pulses raises new challenges, including possible sample damage induced by shock waves that are launched by preceding pulses in the sample-carrying jet. We explored this matter with an X-ray-pump/X-ray-probe experiment employing haemoglobin microcrystals transported via a liquid jet into the XFEL beam. Diffraction data were collected using a shock-wave-free single-pulse scheme as well as the dual-pulse pump-probe scheme. The latter, relative to the former, reveals significant degradation of crystal hit rate, diffraction resolution and data quality. Crystal structures extracted from the two data sets also differ. Since our pump-probe attributes were chosen to emulate EuXFEL operation at its 4.5 MHz maximum pulse rate, this prompts concern about such data collection.
Highlights
X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology
The undisputed benefits of XFELs for structural biology are tied to unique experimental challenges: upon forming a diffraction image, the XFEL pulse annihilates its target, a process dubbed “diffraction before destruction”[10]
Designed to provide up to 27,000 pulses per second, this increases the number of pulses per second by a factor of 225 or more compared to previous XFELs
Summary
X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology. The damaging effect of a pump-pulse-induced shockwave, as indicated by reduced diffraction quality of our haemoglobin microcrystals, is consistent with a stochastic rearrangement of the crystallized molecules or unit cells affecting the order of the crystalline lattice.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
