Effect of withdrawing long days from male American tree sparrows (Spizella arborea): implications for understanding thyroid-dependent programming of seasonal reproduction and postnuptial molt.

Abstract

In previous studies, we withdrew thyroid hormones by thyroidectomy before, at, or after the onset of photostimulation and showed that male American tree sparrows (Spizella arborea) are programmed for seasonal reproduction and postnuptial molt by or before Week 3 on long days. In this corollary study, we withdrew long days before or after the control circuits had been programmed. After 1 day to 4 wk on long days, groups of thyroid-intact males were returned to short days until Week 7, when they were moved to constant light and evaluated for photosensitivity or photorefractoriness and postnuptial molt. Long-day controls held 7 wk on long days showed robust testicular growth through Week 6 and then spontaneous testicular regression. Testes of short-day controls and of males photostimulated for 1 day remained small. In all other groups, photostimulation induced testicular growth, which gave way to regression during exposure to short days. Long-day controls tested photorefractory at Week 7 and initiated molt by Week 10. All other groups tested photosensitive and did not molt. Our demonstration that long days are required for expression of seasonal reproduction and postnuptial molt in thyroid-intact male tree sparrows previously programmed for these events suggests that long days create a milieu that is permissive for expression.