The photoperiodic control circuit in euthyroid American tree sparrows (Spizella arborea) is already programmed for photorefractoriness by week 4 under long days.

Abstract

The aim of this investigation was to determine whether spontaneous testicular regression in male American tree sparrows (Spizella arborea) that were thyroidectomized at week 4 of photostimulation manifests photorefractoriness, as it does in chronically photostimulated euthyroid controls. On the basis of our demonstration that exogenous thyroxine stimulates the gonads only when recipient birds are photosensitive, male tree sparrows were thyroidectomized at week 4 of photostimulation (20 h light:4 h dark) and given thyroxine periodically to assay for photosensitivity. When initiated at weeks 4, 7, 10, 13 and 16 of photostimulation, thyroxine replacement therapy had no effect on testis size until week 16, when most recipient birds showed robust testicular growth. The inductive effect of exogenous thyroxine at week 16 confirms that chronic thyroidectomy dissipates photorefractoriness and simulates the effect of short days. The failure of replacement thyroxine to halt spontaneous testicular regression between week 7 and week 13 establishes that spontaneous testicular regression after thyroidectomy manifests photorefractoriness. Moreover, the failure of replacement thyroxine to induce testicular growth between week 4 and week 7 indicates that by week 4 of photostimulation, at least 3 weeks before photoinduced testicular growth ends, male tree sparrows are programmed for photorefractoriness. This conclusion is strengthened by the finding that thyroidectomy at week 4 of photostimulation does not uncouple photorefractoriness and postnuptial moult, which in euthyroid tree sparrows are tightly linked. In another experiment, photosensitive thyroid-intact tree sparrows were moved from 8 h light:16 h dark to 20 h light:4 h dark and given exogenous thyroxine or vehicle through week 6 of photostimulation. Exogenous thyroxine augmented testicular growth.