Abstract
Gynura procumbens also recognized as Sambung Nyawa is a type of the herbs found in Malaysia, Indonesia and Thailand. It has been consumed as ‘ulam’ by whom that aware of its potential as medicinal remedy and some even drink it as tea for theuraphatic effect. Supercritical fluid extraction is a fast technique to extract valuable compounds from plant parts especially from leaves and seeds. A preliminary study on the effect of solvent water ratio towards yield of Gynura procumbens is conducted at pressure of 20 MPa, temperature of 70 °C, static time of 15 minutes, ethanol as the co solvent and flow rate of 4 ml/min. The time of extraction is 60 minutes. The solvent water ratio was set as follows: 100% ethanol, 75% ethanol and 50% ethanol. The yield obtained are 1.8267%, 0.5933% and 0.9733% for 100%, 75% and 50% ethanol respectively. The extract is then further analysed using high pressure liquid chromatography to determine valuable compounds extracted within the stipulated time at different solvent water ratio. Reversed phase high performance liquid chromatography separation was done using 0.1% phosphoric acid: acetonitirile as eluent in mobile phase to determine flavonoid compounds.
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