Abstract
Objective To explore a protocol for prolonging the in vitro preservation of red blood cells (RBCs)for biological tests.Methods The fresh blood of healthy adult was preservated in the well-established blood eell preservation solutions(CPDA and the Alserver's solution).After the preservation solutions were added by Vitamin E as an additive,the RBC-SOD,RBC-ATP,free hemoglobin(Free Hb)were examined every 7 days in 56-day experimental period.Results The average lifespan of RBCs had risen from 28 days to 42 days in CPDA+Vitamin E and from 7 days to 21 days in Alserve'S solution+Vitamin E respectively at CC.However,the preservation effects of two kinds of solutions are the same in initial 7 days.Conclusion Vitamin E can significantly enhance the protection effects of well-established erythroeyte prescrvation solutions.It is recommended that valuable RBCs sample can be preserved for short period of time in the simpled-component Alserve's with the additive Vitamin E.and be long-term saved with the balanced CPDA and Vitamin E. Key words: red cell preservation; CPDA; Alserve'S; Vitamin EEffect of Vitamin E on Human Erythrocyte Preservation Solutions,LIU Jian,LJ Xiao-mian
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