Abstract
Abstract The modification of radiosensitivity of barley seed with 1.75 × 10−3 M and 3.8 × 10−3 M concentrations of caffeine and ascorbic acid during irradiation in oxygenated and oxygen-free hydration medium was studied at 25 and 37°C, respectively. Both concentrations of caffeine and ascorbic acid afford protection against oxic radiation damage which is maximal at 25°C. Caffeine effectively potentiates the anoxic component of damage but ascorbic acid has no influence at all. at 25°C there is no concentration-dependent effect of caffeine or ascorbic acid. At 37°C, there is no effect, whatsoever, of either concentration of ascorbic acid, whereas caffeine dramatically potentiates the radiaiton damage under both oxygenated and oxygen-free conditions, and the magnitude of potentiation is concentration-dependent. The possible reactivity of caffeine and ascorbic acid towards the precursors of oxygen-dependent and -independent components of damage in determining the mode and magnitudes of modification is discussed briefly.
Published Version
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