Effect of Various Temperatures on Bletillae Rhizoma Polysaccharide Extraction and Physicochemical Properties

Xiaoyan Long,Quan Yan,Xinyue Liu,Xuegang Luo,Linwei Peng

doi:10.3390/app9010116

Xiaoyan Long, Quan Yan + Show 3 more

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https://doi.org/10.3390/app9010116

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Abstract

Six fractional polysaccharides were prepared by water extraction and alcohol precipitation under controlled temperature from bletillae rhizoma, a traditional Chinese medicine. Based on this, yields of bletillae rhizome polysaccharides (RBPs) were obtained. The extracting temperature impacted the characteristics of the fractional polysaccharides. The fractional polysaccharides were characterized by glucomannan (GM) content, thermal stability, scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, gel permeation chromatography (GPC), and x-ray diffraction (XRD). For the analysis, 2.0% w/v dispersions of the six fractional polysaccharides were prepared and their flow behaviors were evaluated using a rotational rheometer. The results showed that increased extraction temperature led to increased GM extraction yields and extraction rate, but GM content was relative stable (over 90%). The average molecular weight (Mw) of fractional polysaccharides obtained at 30, 40, 50, 60, 70, and 80 °C was 3.598 × 104, 4.188 × 104, 8.632 × 104, 8.850 × 104, 2.372 × 105, and 3.081 × 105 g/mol, respectively. SEM revealed that fractional polysaccharides had a porous structure of different sizes and densities. Thermal analysis, FTIR, and XRD results indicated that extraction temperature affects the structure and moisture content of fractional polysaccharides. All results showed that the extraction temperature has an obvious impact on the morphology, molecular weight, and polydispersity of the RBPs. This simple process is a promising method for the preparation of fractional polysaccharides.

Highlights

Polysaccharides collected from biomass continue to attract the attention of researchers due to their apparent advantages, such as low cost, biocompatibility, and nontoxicity [1,2,3]
The prepared crude bletillae rhizoma flour (RBF) should be purified. It was purified by water extraction and alcohol precipitation under controlled temperature to give the pure polysaccharide fraction RBP
From the results obtained our study,0.17 we can7.99 conclude that the bletillae rhizome (RBP30–80) could be prepared by water extraction and alcohol precipitation under

Summary

Introduction

Polysaccharides collected from biomass continue to attract the attention of researchers due to their apparent advantages, such as low cost, biocompatibility, and nontoxicity [1,2,3]. They have many uses in the pharmaceutic field and the food and chemical industries as thickeners, binders, and gels, since they can form high-viscosity solutions in aqueous systems [4,5]. Reichb.f.), is a kind of traditional medicine material originally documented in Chinese medical classics such as the Shen Non. Ben Cao Ging [6]. The backbone of polysaccharides from bletillae rhizome (RBP) is mainly composed of (1→2)-linked α-D-mannopyranose and (1→4)-linked β-D-glucopyranose residues [8]

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