Effect of vanadate on the glucose-1,6-bisphosphate synthase and glucose-1,6-bisphosphatase activities of phosphoglucomutase

Abstract

Phosphoglucomutase, in addition to catalyzing the interconversion of glucose 1-P and glucose 6-P, catalyzes both the synthesis of glucose 1,6-P 2 from glucose monophosphate and either fructose 1,6-P 2 or glycerate 1,3-P 2, and the hydrolysis of glucose 1,6-P 2. Vanadate inhibits the mutase activity, activates the synthase activities, and does not affect the phosphatase activity. These effects suggest that the “exchange” step postulated for the phosphoglucomutase pathway is specifically inhibited by vanadate.