Abstract
The effect of UVA (315–400 nm) irradiation on Echinaceae purpureae interactions with free radicals was examined by the use of electron paramagnetic resonance (EPR) spectroscopy. The changes of antioxidant properties of E. purpureae with time of UV irradiation from 10 to 110 min (10 min steps) were determined. DPPH as the paramagnetic reference was used in this study. Changes of EPR signals of the reference after interactions with nonirradiated and UV-irradiated E. purpureae were detected. Interactions of the tested E. purpureae samples caused decrease of the EPR signal of DPPH as the result of its antioxidant properties. The decrease of the amplitude of EPR line of DPPH was lower for interactions with UV-irradiated E. purpureae. EPR examination confirmed antioxidant properties of E. purpureae. The weaker antioxidant properties of E. purpureae after UV irradiation were pointed out. E. purpureae should be storage in the dark. The tests bring to light usefulness of electron paramagnetic resonance with microwave frequency of 9.3 GHz (an X-band) in examination of storage conditions of pharmacological herbs.
Highlights
The ability of the food and pharmacological substances to interactions with free radicals is their important property (Pawłowska-Goral et al, 2013; Rzepecka-Stojko et al, 2012)
The effect of UVA (315–400 nm) irradiation on Echinaceae purpureae interactions with free radicals was examined by the use of electron paramagnetic resonance (EPR) spectroscopy
Nonirradiated and UV-irradiated E. purpureae reveal antioxidant properties; it interacts with free radicals and as the result, it causes decrease of EPR signal of the paramagnetic reference—DPPH in ethyl alcohol solution
Summary
The ability of the food and pharmacological substances to interactions with free radicals is their important property (Pawłowska-Goral et al, 2013; Rzepecka-Stojko et al, 2012). The results of therapy depend on quenching of free radicals in living organism. Free radicals are responsible for a lot of negative effects in organism, and their inactivation is needed. Free radicals have unpaired electrons, which cause major biochemical reactions and destroy the structures in cells. The substances used in medicine should not contain free radicals, and they should be antioxidants. Pharmacological species as antioxidants react with free radicals, which loss their unpaired electrons and become diamagnetic. The activity of diamagnetic molecules is lower than paramagnetic free radicals, the risk of modification of chemical structures in tissues decreases, and their functions are not destroyed (Jaroszyk, 2008; Bartosz, 2006)
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