Effect of uterine immunomodulation on hematobiochemical parameters in cyclic non-breeding cows

Saraswat Sahoo,Arpita Padhy,Srinibas Das,Debendra Narayan Mohanty

doi:10.14202/vetworld.2014.816-820

Abstract

Aim: To study the effect of uterine immunomodulation on hematobiochemical parameters and total immunoglobulin concentration in cyclic non-breeding cows. Materials and Methods: Twenty-one repeat breeding cows around Bhubaneswar area were screened by white side test to detect and treat the endometritis and were assigned to three treatment protocols with an equal number of seven animals in each group. Cows in control group were administered with 50 ml of normal saline while treatment Group I animals were given single intrauterine infusion of 20 ml of fresh colostrum and treatment Group II animals received nonpathogenic Escherichia coli in 10 ml sterile saline. The blood samples were collected from all the experimental animals, and hematobiochemical parameters and total immunoglobulin concentration were estimated. Results: A high significant difference (p<0.01) was accounted in lymphocyte count of E. coli treated group within different days of sampling. Analysis of variance recorded a highly significant difference with neutrophil percent in E. coli lavaged cows. In colostrum treated group monocyte count showed a significant difference (p<0.01) between 0 and 14 th day of sampling. The analysis of hematocrit values did not show any significant difference apart from the erythrocyte sedimentation rate parameter in the colostrum infused group with the highest significant (p<0.01) variation being observed between 7 th and 14 th day of sampling. The analysis of aspartate amino transferase values in the colostrum lavaged cows revealed a significant difference, but that of alanine amino transferase values did not show any significant difference. Comparison of immunoglobulin values for different days in all the treatment protocol revealed a highly significant (p<0.05) difference within various days of sampling. Conclusion: In the present study, the local immunomodulation by different agents have been highlighted and which indicated potentiation of uterine immunity by different drug that might serve as a new direction of treatment to uterine diseases. The scope of research in the future should be widened by considering a larger population for validation.

Highlights

In a recent past, the incidence of infertility has relatively increased with a consequent reduction of productivity of farm animals
Cows in control group were administered with 50 ml of normal saline (I/U) while Group II animals received 20 ml of fresh colostrum (I/U) and Group III animals were infused with 10 ml of non-pathogenic Escherichia coli in sterile saline (I/U)
The eosinophil percent remained within the range of 4.71-8.14, 6.86-10.43 and 5.86-9.00 in normal saline, colostrum and E. coli treated cows at 0, 7, 14 and 21 day of sampling and showed non-significant change

Summary

Introduction

The incidence of infertility has relatively increased with a consequent reduction of productivity of farm animals. It is accepted that bovine genital infection either specific or non-specific in nature, accounts for a large number of pregnancy failure in cows [1]. These infections alter the uterine environment resulting in impairment of sperm transport, sperm death and hostile environment to the subsequent development and maintenance of conceptus, leading to their death, there by affecting their fertility. Estimation of blood parameters can give an idea about severity of infection and we can check it either by use of conventional antibiotics or by non-conventional uterine immunomodulators.

Objectives

Methods

Results

Discussion

Conclusion