Effect of Certain Immunomodulators on Uterine Microbiology in Cyclic Non Breeding Cows

Abstract

Immunomodulators, uterine microbiology, Cow A total number of twenty one repeat breeding cows selected after meticulous screening based on Whiteside test and Laing’s criteria then allotted randomly into three equal groups (n=7). Cows in control group were lavaged with intra-uterine with 50 ml of normal saline, in group I 20 ml of fresh colostrum and in group II infusion with 10 ml of non-pathogenic E.Coli in sterile saline as intra uterine medication were given. Bacterial isolates were collected in pre-treatment stage (day0) and post treatment stage (day 21) and their concentrations (cfu/ml) were compared.In the pre treatment stage, the predominant bacterial flora and their frequency in animals constituted Klebsiella spp , Staphylococcus spp , E.coli , Enterococcus fecalis , Streptococcus spp , Proteus spp and Bacillus spp . In the post treatment sampling after twenty one days, following different drug treatments revealed inhibition of many bacterial isolates which were identified in the pre-treatment period. Klebsiella spp. was found only in normal saline group whereas it was absent in colostrum and E.coli treated group. Similarly Staphylococcus spp. was identified from a single case in colostrum treated group whereas the other two treatment regimen did not reveal their presence. Similar bacterial inhibition against Staphylococcus spp. was marked in normal saline and E.coli. The presence of non pathogenic E.coli was evident in all the treatment groups at post treatment period, indicating either their reinfection or due to its natural presence in the reproductive tract. The cows in control group projected a first insemination conception rate of 42.86%, in group – I recorded a pregnancy rate of 57.14% and in group – II three cows became pregnant (42.86%) out of seven inseminations.