Abstract
1. 1. Optimum conditions were studied tor the determination of RNA polymerase activity in nuclei isolated from Rana catesbeiana tadpole hindlimbs. 2. 2. Tadpole nuclei were tested at 15°C in the presence of spermicline (1.5 mM) bovine serum albumin (1.0%) and a high concentration of nucleoside triphosphates (1.0 mM). 3. 3. Tadpole nuclei exhibited a 60–70% higher total RNA polymerase activity with maximum activity of RNA polymerase I at 4 hr and RNA polymerase II at 8 hr after triiodothyronine injection. 4. 4. The results support a nuclear mechanism for the differentiation of tadpole hindlimbs induced by triiodothyronine.
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