Abstract
The objective of this work was to assess the influence of compressed CO 2 and propane treatment on the specific activity of partially purified d-hydantoinase from adzuki bean ( Vigna angularis). The lyophilized protein was obtained through the precipitation of proteins with (NH 4) 2SO 4 followed by lyophilization of the precipitate. The d-hydantoinase activity was determined using hydantoin as substrate at 40 °C and pH 9.0. The effect of temperature, pressure, and exposure time on the solubilized and lyophilized partially purified d-hydantoinase extract in compressed CO 2 and propane were evaluated. Treatment of this extract from adzuki bean ( V. angularis) in compressed CO 2 and propane showed good stability of this enzyme in the two solvents. The resolubilized enzyme extract presented important activity losses with compressed CO 2 treatment and better stability when treated with compressed propane.
Published Version
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