Abstract
A GUS marker was used in earlier experiments to show that crown gall resistant Vitis genotypes show significantly lower rates of transformation by A. vitis than the crown gall susceptible Vitis vinifera. In recent experiments a reporter gene (lacZ) fused with the virB promoter was used to determine whether the lower frequency of transformation resulted from reduced induction of Agrobacterium vitis virulence (vir) genes. With some A. vitis strains, addition of medium conditioned with grape tissue enhanced vir induction by the known inducer acetosyringone and significant vir induction also resulted without addition of acetosyringone when grape tissue was included in the induction medium. Enhancement of vir induction by grape extracts was not correlated with genotypic susceptibility to crown gall.
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