Abstract
The heterozygous (Su/su) aurea mutant, the wild-type (su/su) and albino (Su/su) varieties of John Williams Broadleaf tobacco ( Nicotiana tabacum L.) were grown as shoot cultures on artificial medium. Leaf extracts of the plants were utilized in determining the specific activities of ribulose 1,5-biphosphate (RuBP) carboxylase/oxygenase (EC 4.1.1.39) and glutamate synthase (EC 1.4.7.1). The specific activity level of RuBP carboxylase/oxygenase was found to be about 75% lower in the albino variety than in heterozygote or wild type plants, which had approximately similar activities. Levels of glutamate synthase activity were similar in all three types. Glutamate was synthesized at similar rates by chloroplasts isolated from all three varieties. It was found that mitochondria present in crude chloroplast preparations could compensate for the absence of α-ketoglutarate (α-KG) omitted from assay mixtures for the estimation of glutamate production by the chloroplastic glutamate synthase system.
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