Effect of the Steroidal Saponins of &lt;em&gt;Fructus tribuli&lt;/em&gt; on Growth of Three &lt;em&gt;Lactobacillus acidophilus&lt;/em&gt;

He Chen,Qi Ma,Guowei Shu,Tao Qin,Zhe Ji

doi:10.19026/ajfst.6.3043

Abstract

Effect of the steroidal saponins of Fructus tribuli on growth of Lactobacillus acidophilus LA04, LA05 and LA06 was studied by measuring optical density at 600 nm (OD600) and pH using MRS media as the control. The addition of steroidal saponins (w/v) was 0.05, 0.10, 0.15, 0.20 and 0.25%, respectively. Results were as follows: addition of steroidal saponins of Fructus tribuli could promote the growth of Lactobacillus acidophilus LA04, LA05 and LA06 and the optimum concentration of steroidal saponins was all 0.25% for the three strains.

Highlights

Lactobacillus acidophilus, which is Gram-positive, non-spore-forming rods with rounded ends that occur singly, in pairs and in short chains, is one of the most commonly recognized species of the genus Lactobacillus and was first isolated from the intestinal tract and vagina of humans and animals (Gopal, 2011)
We found that the hot water extract of Fructus tribuli can promote the growth of Lactobacillus acidophilus LA09 (Shu et al, 2011)
Effect of steroidal saponins on growth of Lactobacillus acidophilus LA04: Effect of different concentrations of Fructus tribuli steroidal saponins on the growth of Lactobacillus acidophilus LA04 showed in Fig. 1 to 3

Summary

Introduction

Lactobacillus acidophilus, which is Gram-positive, non-spore-forming rods with rounded ends that occur singly, in pairs and in short chains, is one of the most commonly recognized species of the genus Lactobacillus and was first isolated from the intestinal tract and vagina of humans and animals (Gopal, 2011). Lactobacillus acidophilus has been shown to play an important role in promoting and maintaining human health and is accepted as probiotics (Salminen et al, 1998; Guarner and Schaafsma, 1998). The health benefits include reduction of gastrointestinal symptoms in lactose-intolerant individuals, relief from symptoms of constipation, treatment of infantile diarrhea, prevention of travellers’ diarrhea and activity against Helicobacter pylori (Choi et al, 2011; Tabbers et al, 2011; Rerksuppaphol and Rerksuppaphol, 2010; Jain et al, 2010). The steroidal saponins of Tribulus terrestris are considered to be one of the factors responsible for biological activity of products derived from this plant and many pharmaceutical preparations and food supplements based on the saponin fraction from this plant are on sale worldwide (Dragomir et al, 2008)

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Results