Abstract

The inhibitory effect of 2″- O-glycosyl isovitexin (2″- O-GIV) isolated from green barley leaves on superoxide and hydroxyl radical generation was investigated using electron spin resonance spectroscopy. 2″- O-GIV exhibited dose-related inhibitory activity both towards superoxide generation and hydroxyl radical generation. 2″- O-GIV inhibited superoxide formation by 97% at a level of 25 m m and inhibited hydroxyl radical formation by 91% at a level of 500 μ m. The results suggest that the inhibitory activity of 2″- O-GIV is due to trapping radicals.

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