Effect of the French oak wood extract Robuvit on markers of oxidative stress and activity of antioxidant enzymes in healthy volunteers: a pilot study.

Martina Horvathova,Magdalena Vavakova,Peter Rohdewald,Zdenka Durackova,Jana Muchova,Zuzana Orszaghova,Peter Sabaka,Lucia Laubertova

doi:10.1155/2014/639868

Abstract

We examined in vitro antioxidant capacity of polyphenolic extract obtained from the wood of oak Quercus robur (QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found an in vitro antioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasma in vivo.

Highlights

The French oak wood extract Robuvit (Horpag Research Ltd.) is a registered proprietary water extract obtained from the wood of Quercus robur (QR)
We examined in vitro antioxidant capacity of polyphenolic extract obtained from the wood of oak Quercus robur (QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers
Basic biochemical parameters were investigated in fasting venous blood in examination periods 0, 4, and 6: bilirubin, glucose, GMT, ALP, AST, ALT, UA, TP, TCh, TAG, HDLchol, LDL-chol, and VLDL-chol

Summary

Introduction

The French oak wood extract Robuvit (Horpag Research Ltd.) is a registered proprietary water extract obtained from the wood of Quercus robur (QR). The plant belongs to the plant family Fagacae, genus Quercus. The oak wood used for Robuvit originates exclusively from oak trees grown in France. Oak wood contains a specific profile of tannins named roburins that are part of the ellagitannins (ETs). Robuvit is standardized and specified to contain at least 20% of roburins (A, B, C, D, and E) including grandinin. The two most abundant ETs in the Robuvit are stereoisomers vescalagin and castalagin, which were originally isolated and described by Mayer et al [1]

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