Effect of the extraction technique on the recovery of bioactive compounds from eucalyptus (Eucalyptus globulus) wood industrial wastes

Abstract

Eucalyptus (Eucalyptus globulus) wood veneer trimmings, a waste product from the wood board industry, were studied as source of bioactive compounds. Two extraction techniques, maceration in an orbital bath and microwave-assisted extraction (MAE), were compared. In the conventional one, the effect of solvent (water, MeOH, EtOH, 50% MeOH and 50% EtOH), temperature (50 and 75°C) and particle size (ground or unground material) on extraction yield and extract properties (total phenols content (TPC) and FRAP, DPPH and ABTS antioxidant activities) were analyzed. Extraction yield increased with increasing temperature and with reducing particle size. However, extract properties decreased when temperature was increased. Extracts obtained with EtOH at 50°C showed the highest antioxidant properties. MAE experiments were planned according to an incomplete 33 factorial design to study the influence of temperature (50–70°C), liquid–solid ratio (5:1–10:1mL/g) and time (5–15min) on extraction yield and extract properties. The optimal condition selected were 65°C, liquid–solid ratio of 8.8:1(mL/g) and 10min. Comparing both techniques, maceration led to the extract with the best antioxidant properties, but MAE allowed to reduce significantly the extraction time. The aqueous extracts obtained under conventional extraction were able to inhibit the growth both bacteria and fungi. Gallic acid esters of glucose, gallic acid, ellagic acid and small proportions of quercetin-3-O-rhamnoside were found in the aqueous and ethanolic extracts.