EFFECT OF THE AMINO ACIDS UPON LYTIC ACTIVITY OF BACILLUS AMYLOLIQUEFACIENS SUBS P. PLANTARUMSTRAINS

Abstract

The aim of the work was to study the lytic activity of probiotic strains of B. amyloliquefaciens subsp. plantarum and the possibility of using amino acids for its stimulation. Methods. The research objects were B. amyloliquefaciens subsp. plantarum UCM B-5139, UCM B-5140 – the basis of therapeutic and prophylactic preparation endosporin. The amino acid (DL-) and dry hydrolyzate mixture of amino acids were used. The lytic enzyme activity of bacilli was determined by the turbidimetric method and expressed as the percentage decrease in the optical density of live cell suspension of the test cultures. Results. Stimulation of the growth and lytic activity of studied bacilli strains against Gram negative bacteria cells by the mixtures of amino acids was installed. However, the separate amino acids such as arginine, alanine, tryptophan, histidine and phenylalanine stimulated the growth of B. amyloliquefaciens subsp. plantarum and the synthesis of lytic enzymes were more effective. The degree of destruction of the E. coli cell walls increased more than 30–100% and S. aureus in 2.3–4 times. Conclusion. High lytic activity of bacilli characterizing their antimicrobial properties, provided spore-forming bacteria harmless for warm-blooded may be no less important than their antagonistic activity against pathogenic and conditionally pathogenic microorganisms in the selection of strains for development of probiotics.