Abstract
Purpose: To evaluate the effect of tangeretin on ovalbumin (OVA)-induced allergic respiratory asthma in Swiss albino mice. Methods: Mice were injected intraperitoneally with 50 μg OVA dissolved in water and 1 mg of aluminium hydroxide (alum) on the first day, and on day 12. On days 22, 26 and 30, the mice were exposed to 1 % OVA for 30 min. Mice in the asthma control group received saline via the same route. Tangeretin (50 or 100 mg/kg body weight) was given orally from day 1 to day 30. On the day of sensitization and challenge, tangeretin (50 or 100 mg/kg body weight) was injected 30 min prior to the treatment. Bronchial function, degree of inflammation, broncho-alveolar lavage fluid (BALF), levels of IL-4, IL-5, IL-13 and IFN-γ in BALF, serum total IgE, OVA-specific IgE, IgG1 and IgG2a, as well as histopathology of lungs were determined. Results: Tangeretin administration led to significant reduction in airway sensitivity, and significant decreases in leukocyte, macrophage, and eosinophil counts (p < 0.05). It also brought about significant decreases in the levels of the pro-inflammatory cytokines (IL-4, IL-5 and IL-13) in both BALF and serum (p < 0.05). In addition, tangeretin reversed the OVA-induced histopathological anomalies in the lungs. Conclusion: These results demonstrate that tangeritin exerts protective effects against OVA-induced allergic respiratory asthma in Swiss albino mice, and that the drug can potentially be developed for the management of allergic asthma. Keywords: Allergic asthma, Inflammation, Cytokines, Ovalbumin, Tangeretin
Highlights
Asthma is a severe, long-lasting inflammatory ailment characterised by reversible respiratory airway congestion
Correlations were determined by one-way analysis of variance (ANOVA), followed by Tukey's test where appropriate
The results showed that exposure of OVA-treated mice to tangeretin inhibited Th2 cytokine secretions (IL-4, IL-5 and IL-13) in a dose-dependant fashion, and increased the secretion of Th1 cytokine in broncho-alveolar lavage fluid (BALF)
Summary
Long-lasting inflammatory ailment characterised by reversible respiratory airway congestion. In the present study which was based on the extensive beneficial properties of tangeretin, the anti-inflammatory and immunomodulatory activities of tangeretin were investigated in a mouse model of allergic asthma. The following chemicals and reagents were commercially obtained: OVA and 100 % pure, endotoxin-free tangeretin (Simcere Doyea Pharmaceutical Co., China); bovine serum albumin (BSA, Atlanta Biologicals, Lawrenceville, GA, USA)); pancuronium bromide (Xi'an Miracle Biotechnology Co., Ltd.); antidinitrophenol (anti-DNP) and IgE mab (Atlanta Biologicals, Lawrenceville, USA); peroxidaselabeled anti-mouse IgE and goat IgG polyclonal antibody After blocking with 1 % Block-Ace, the reference and serum dilutions were left to stand for one hour and treated with peroxidaselabeled anti-mouse immunoglobulin E goat IgG polyclonal antibody. Serum ovalbumin-specific IgG1 was assayed by coating the microtiter plates with goat anti-mouse IgG1 polyclonal antibody (2 μg/mL). For anti-ovalbumin IgG2a, the limit of detection was 3 ng/mL
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