Abstract
Objective To investigate the antiasthmatic effects of Sanglong pingchuan decoction (SLPCD) and to explore its mechanisms of action. Methods The serum, bronchoalveolar lavage fluid (BALF), and lung tissues from OVA-induced allergic asthma mice were collected 24 h after the last administration. Lung pathological changes were observed by H&E staining. The inflammatory cells in BALF were counted by flow cytometry. The levels of total IgE in serum and cytokines in BALF were determined by ELISA. The expression levels of cytokine mRNA in lung were assayed by qRT-PCR. Results SLPCD significantly inhibited airway inflammation, reduced inflammatory cells in BALF, reduced the levels of total IgE in serum and Th2 cytokines (IL-10 and IL-13) in BALF, and downregulated the mRNA expression levels of Th2 cytokines (IL-4, IL-5, IL-10, and IL-13) in lung of asthmatic mice. However, SLPCD remarkably elevated the level of Th1 cytokine IFN-γ in BALF and upregulated the mRNA expression levels of Th1 cytokines (IL-2 and IFN-γ) in lung of asthmatic mice. Conclusion SLPCD could attenuate airway inflammation and alleviate the pathogenesis in asthma mice through inducing a balanced Th1/Th2 response and could act as an effective drug for treatment of asthma.
Highlights
Chronic respiratory disease that is characterized by bronchial hyperresponsiveness, airway inflammation and remodeling, airflow obstruction, and infiltration of different kinds of inflammatory cells induced by cytokines and other mediators [1, 2]
To provide experimental basis for the clinical application of Sanglong pingchuan decoction (SLPCD) in treating asthma and to explore its mechanisms of action, using OVA-induced allergic asthma mouse model, the anti-inflammatory effects of SLPCD were investigated by histological examination, enumeration of immune cells in bronchoalveolar lavage fluid (BALF), quantization of total IgE in serum, cytokine in BALF, and the mRNA expression of cytokine in lung tissues
The purified anti-mouse CD16/CD32 (Fc Receptor block), Ly-6G–FITC, F4/80 antigen PECy5, Ly-6C-APC, Fc epsilon Receptor 1 alpha (FceR1)–APC, CD117-PE-Cy5 (c-Kit, clone: 2B8), and siglec-F-PE antibodies were purchased from eBioscience, Inc., San Diego, CA, USA
Summary
To investigate the antiasthmatic effects of Sanglong pingchuan decoction (SLPCD) and to explore its mechanisms of action. The levels of total IgE in serum and cytokines in BALF were determined by ELISA. The expression levels of cytokine mRNA in lung were assayed by qRT-PCR. SLPCD significantly inhibited airway inflammation, reduced inflammatory cells in BALF, reduced the levels of total IgE in serum and Th2 cytokines (IL-10 and IL-13) in BALF, and downregulated the mRNA expression levels of Th2 cytokines (IL-4, IL-5, IL-10, and IL-13) in lung of asthmatic mice. SLPCD remarkably elevated the level of Th1 cytokine IFN-γ in BALF and upregulated the mRNA expression levels of Th1 cytokines (IL-2 and IFN-γ) in lung of asthmatic mice. SLPCD could attenuate airway inflammation and alleviate the pathogenesis in asthma mice through inducing a balanced Th1/Th2 response and could act as an effective drug for treatment of asthma
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