Effect of superoxide dismutase, catalase, and glutathione reductase supplementation on cryopreservation of Black Bengal buck semen.

Abstract

The present experiment was carried out with the objectives to study the effects of antioxidants superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH) on cryopreservation of Black Bengal buck semen. Semen ejaculates (n = 60) were collected from eight bucks by artificial vagina method and diluted with Tris citrate egg yolk glycerol extender. To study the effect of antioxidants, SOD was added @ 0, 100, and 150IU/ml; CAT was added @ 0, 200, and 400IU/ml while GSH was added @ 0, 1, and 2mM of diluted semen. Semen samples were equilibrated and vapor frozen in liquid nitrogen. Semen samples were evaluated after 48h of storage for post thaw in vitro characters such as motility, viability, functional membrane integrity, and acrosome integrity. Semen extenders supplemented with SOD @ 100 and 150IU/ml and GSH @ 1 and 2mM had a higher (p < 0.01) number of motile cells, viable cells, HOST reacted cells, and acrosome intact cells than their respective controls. Further, semen extenders added with catalase @ 200 and 400IU/ml had more (p < 0.05) number of viable, HOST reacted cells and significantly higher (p < 0.01) acrosome intact sperm cells than its control group. It can be concluded that supplementation of antioxidants SOD, GSH, and CAT had a beneficial effect on cryopreservation of Black Bengal buck semen.