Abstract
Abstract Objective To assess the effect of different seasons on sperm motility, viability, total sperm abnormality, acrosomal and plasma membrane integrity, antioxidant profiles such as superoxide dismutase (SOD) and catalase (CAT), enzymatic profiles such as aspartate amino transaminase (AST), lactic acid dehydrogenase (LDH) and biochemical profiles such as total cholesterol in Indian breed, Tharparkar bull. Methods Total numbers of 60 ejaculates from 3 bulls were collected through artificial vagina method twice a week during summer and winter season (30 ejaculates from each season). The semen samples were pooled and diluted with the standard TEYC extender and these seminal, biochemical parameters were studied at fresh, pre- freeze and post thaw stage of semen cryopreservation. Results Seminal parameters such as volume, plasma membrane integrity and biochemical parameters such as SOD at fresh semen, acrosomal integrity, LDH, AST and total cholesterol at pre–freeze level and acrosomal integrity, SOD at post thaw stage of cryopreservation were differed significantly ( P P P P P P P Conclusion It can be concluded that cryopreservation of Tharparkar bull semen in summer and winter season do not show any definite pattern in relation to seminal and biochemical profile changes and the semen can be cryopreserved in both seasons throughout the year in this prestigious Indian breed of cattle.
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