Abstract
In this study, the effect of sucrose on the neutral lipid profile of adventitious root cultures of Stevia rebaudiana was evaluated. The cultures were obtained employing a roller bottle system. In this system, Schott-type flasks were used, which contained Murashige and Skoog liquid medium at 33.3% strength (MS/3) supplemented with 30, 60, and 80 g L-1 of sucrose, respectively, and 10.7 mM 1-naphthaleneacetic acid (NAA). The spectroscopic analyzes showed that the portion of polyunsaturated fatty acids (PUFAs) was highest in roots treated with 30 g L-1 of sucrose. The spectrometric analyzes showed that the palmitic acid was found to be present in relatively higher amounts in the roots submitted to the MS/3-30 g L-1 (31.9%) and MS/3-60 g L-1 (29.5%) sucrose treatments, and lower in the treatment with MS/3-80 g L-1 (28.8%) of sucrose. Also, the treatment using 30 g L-1 of sucrose was the best for obtaining unsaturated fatty acids (UFAs) in the culture, with a relative percentage of 62.9%. Our results indicate that the MS medium that received 30 g L-1 of sucrose induced a lesser abiotic stress condition, which favored PUFAs production in the adventitious root cultures of S. rebaudiana.
Highlights
Stevia rebaudiana (Bertoni) Bertoni is a species of perennial shrub belonging to the family Asteracea (Asterales)
3.1 Effect of sucrose on the accumulation of biomass in adventitious root cultures in vitro of S. rebaudiana One of the main arguments to invest time and effort into the search for biotechnological alternatives for the production of bioactive metabolites is because the yields of these are normally low in the wild specimens, and often only found in specific plant tissues or species
The elicitation technique, used to enhance the synthesis of metabolites in order to ensure plant survival, persistence or competitiveness, has proven to be a competent approach both in the ex vitro and in in vitro root systems (Tocci et al, 2012; Yin et al, 2012). This bioreactor allows the flow of the culture medium to be uniform within the flasks, allowing the roots to properly absorb nutrients from the medium. This system was successfully used in the adventitious root cultures of S. rebaudiana by Reis et al (2011) and Lopes et al (2016).In this work, the effect of increasing concentrations of sucrose of 30 g L-1, 60 g L-1 and 80 g L-1onroot growth and the production of neutral lipids (NLs) in the adventitious root cultures of S. rebaudiana was evaluated
Summary
Stevia rebaudiana (Bertoni) Bertoni is a species of perennial shrub belonging to the family Asteracea (Asterales) This plant (in Guarani language Ka’ahe’ê) is native to South America (Tavarini, & Angelini, 2013), and popularly known “sweet herb of Paraguay, sweet leaf, candy leaf, or honey leaf” (Soejarto, Kinghorn, & Farnsworth, 1982; Brandle, & Rosa, 1992; Madan et al, 2010). The main producers of Stevia are China and Southeast Asian countries. This plant has been gaining importance since 2011 when steviol glucosides obtained from the leaves were recognized as a sweetener by some important regulatory agencies around the world (Tavarini, & Angelini, 2013). Studies have shown that its roots are a rich source of inulin, a compound used as a prebiotic source (Lopes et al, 2016)
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