Effect of stromal interaction molecular 1 gene on proliferation and apoptosis of prostate cancer PC-3 cells

Abstract

Objective To evaluate the effects of stromal interaction molecular 1 (STIM1) on proliferation and apoptosis of prostate cancer PC-3 cells.Methods STIMI siRNA sequence was designed and constructed into plasmid pGCSIL-GFP vector.The sequence inserted was confirmed by sequence test and polymerase chain reaction (PCR).The efficiency of plasmid pGCSIL-GFP-STIM1 was confirmed by ectopic cotransfection test.Chromic virus STIM1-RNAi-LV was packed.After it was transfected into prostate cancer PC-3 cells,the proliferation,apoptosis and cell cycle of PC-3 cells were evaluated.Results The plasmid vector pGCSIL-GFP-STIMI was confirmed to be correct and effective by sequence examination,PCR and ectopic target test.After the Chromic virus STIM1-RNAi-LV was transfected into PC-3 cells,the proliferation ability in experimental group was apparently reduced as compared with that in control group.After transfection for 5 days,the apoptosis rate in experimental group was ( 29.85 ± 0.86 ) ％,and that in control group was (2.92 ± 0.14) ％ ( P ＜ 0.05 ).At the same time,the number of cells in the stage G1 and S was reduced and that in stage G2/M was increased in experimental group as compared with control group ( P ＜0.05).Conclusion When STIM1 gene was inhibited in prostate cancer PC-3 cells,the proliferation was reduced,the number of apoptotic was increased and the cell cycle statues were changed,indicating that STIM1 may have important role in proliferation and apoptosis of PC-3 cells. Key words: Prostate neoplasm;  Stromal interaction molecular 1;  Apoptosis