Effect of interferon-.BETA. gene engineered human bone mesenchymal stem cells on proliferation and apoptosis of PC-3 cells

Abstract

Objective To investigate the effect of human interferon-β (IFN-β) gene cDNA engineered human bone marrow mesenchymal stem cells (hMSCs) on the proliferation and apoptosis of prostate cancer cell line PC-3.Methods The pCDNA3.1-IFN-β vector containing human IFN-β gene was constructed and transfected into hMSCs by lipofectamine.IFN-β-expressing mesenchymal stem cells (IFN-β-hMSCs) were incubated with PC-3 cells.PC-3 cells were stained by trypan blue and viable cells were counted by hemocytometer respectively at the 1 st,3rd,and 5 th day after co-culture.The apoptosis of PC-3 cells was analyzed by flow cytometry.Results IFN-β-hMSCs could express IFN-3 successfully.Five days after co-culture,the number of viable PC-3 cells in hMSCs,mock-hMSCs,IFN-β-hMSCs groups was ( 10.81 ± 0.33) × 105,(9.90 ± 0.41 ) × 105,and (4.63 ± 0.32 ) × 105 respectively.IFN-β-hMSCs resulted in a significant decrease in PC-3 cells survival as compared with control group (P ＜0.01 ),and apoptosis rate of PC-3 cells was (22.79 ±2.71 )％,which was increased as compared with control group.Conclusion The IFN-β-hMSCs can express IFN-β successfully and inhibit the growth of prostate cancer cells significantly. Key words: Prostate carcinoma; Interferon beta; Mesenchymal stem cell; Gene therapy