Abstract
The present study included examination of (27) human semen sample, forinfertilePatients of cryptozoospermiaresorting tothe fertility center /Al-sader medical city in Al –Najafprovincesfrom November 2011intomay 2012. microscopicexaminationwas usedforCryptozoospermicpatient, and thenwere used three activation techniques (swim up from pellet, density gradient and glass wool column filtration ) As,there are rare studies of cryptozoospermic patients, thus this study was aimed to test the efficiency of someactivation techniques in sperm activation for cryptozoospermia patientby swim up from pellet, density gradient and glass wool column techniques for using inintracytoplasmic sperm injection (ICSI).The results of sperm activation (swim up from pellet, density gradient and glass wool column filtration ) for Cryptozoospermic patients had shown highly quality of sperm in selected toward progressive motilityand normal morphology. In addition to ability for selection of normal morphology sperm and progressive motility percent also glass woolcolumn filtration showan important role in maintenance of sperm concentration before sperm activation in comparison with after activation. The result of swim up from pellet and density gradient revealed a significant decreasing(P<0.05)ofsperm concentration after activation in comparison with before activation. So the result of swim up from pellet, density gradient and glass wool filtrationshowed a significant increasing (P<0.05)of progressive motility and, viabilitypercent and normal sperm morphology percent, but a significant increasing(P<0.05) of non – progressive motility sperm and Round cell after activationcomparedwith beforeactivationof all three different uses techniques In conclusionof studythat sperm activation techniquesrole by swim up from pellet, density gradient and glass wool column techniques for Cryptozoospermia patients giving best results afterICSI processtohavingpregnancy rates and removedto some infertile problem
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