Abstract
IntroductionTo select good-quality sperm for assisted reproductive technology procedures, the best spermatozoa should be elicited. The aim of this study is to compare the impact of sperm selection on human sperm DNA fragmentation and mitochondrial membrane potential by means of density gradient and swim-up techniques. Materials and methodsConventional and functional semen parameters (mitochondrial membrane potential and plasma membrane integrity) in sperm selected by density gradient and swim-up techniques in 14 semen samples were evaluated. ResultsIn the selected neat semen samples, the number of sperm recovered was lower using swim-up as compared to density gradients. By contrast, an increase in progressive motility was observed compared with the initial sample, with no statistically significant difference between both techniques. On the other hand, the swim-up technique was shown to decrease the amount of non-progressive motile sperm recovered, whereas the density gradient technique allowed for a better recovery of the total motile sperm selected. Finally, an increase in the proportion of cells with higher mitochondrial membrane potential and lower DNA fragmentation index was observed. ConclusionThe results of this study demonstrate that sperm selection using the swim-up technique allows for better-quality sperm to be recovered, with a higher sperm cell membrane potential and lower DNA fragmentation. The use of this method is therefore recommended prior to artificial insemination or in vitro fertilisation procedures.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Clinica e Investigacion en Ginecologia y Obstetricia
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.