Effect of some substances chemical on the crystallisation of calcium oxalate in vivo

Abstract

Purpose: Epidemiological data collected during several decades that the majority of stones, up to 80% are composed of calcium oxalate (CaOx). This current study was aimed to look for an alternative treatment by using chemical substances on nephrolithiasic rabbits as a preventive against the development of urinary stone. The conditions of crystallisation and inhibition of oxalate calcium were studied at in vivo and in vitro. The experiment was conducted in normal and calcium oxalate (CaOx) nephrolithiasic abbits during three months. Several parameters were flowed every day including water intake, urinary volume and pH and crystalluria. Objectif: Our objective in this part of work was to determine the chemical inhibitors there act on the states of crystallization of calcium oxalate efficiently. A survey previous to the laboratory showed the in vitro inhibitory power of the chemical substances that we tested on the calcium oxalate. A comparison will be made between the two studies, only in the goal to confirm or to invalidate a possible effect of inhibition. The studied inhibitory substances are: the citric acid, Mg2*, Fe2*, F-. Methods: XVc divided the rabbits aged of 1 mois with a middleweight of 960g in two groups of 6. The second group was considered like witnesses. The two groups are nourished in industrial food. Result: The inhibition capacity of Fluoride, citrate, ferric and Magnesium ion were important on the aggregation and size of oxalate calcium crystals, but didn't inhibit completely this crystalline species.