Abstract
Helminthes have embryotoxic and teratogenic effects to host’s cells and tissues.Investigations were carried out mainly on embryo and fetus in mammals.Blaszkowska, J. (1998); Kadlubowski R. (2000) conducted studies of in chickembryos. Dose-dependent effect is also studied and found that small doses causeembryotoxic effect, while higher doses - teratogenic. Anisakis simplex antigenshave also embryotoxic effect has been found. The aim of our research was toexamine the effect of A. simplex somatic antigen to the development of chickembryos in its experimental introduction in various ways and at different stages ofembryogenesis. For the study chicken embryos were used at different stages ofembryogenesis: 7.5, 10.5 and 12.5 days. Methods for introducing antigen chickenembryos: in the allantois cavity, on-chorion allantois membrane and yolk sac at 0.2ml. The autopsy was performed on embryos 2 and 8 days. The eggs and embryoswere weighed and evaluated the embryonic development. As a result of the firstexperience the greatest change observed by mass, and when administered in thedevelopment of antigen in both yolk sac on day 2 (1.68 ± 0.06, unlike control 2.25± 0.20), and at 8 days ( 9.97 ± 6.21 in the experiment versus 14.89 ± 0.78 in thecontrol), respectively. In the second experiment eggs were used at 1 and 5 dayincubation embryos. The antigen was administered in both cases also in the yolksac at a dose of 0.2 ml. In the second experiment there was a delay in thedevelopment of both cases at autopsy after 2 days in non-incubated eggs missingthe development of, unlike the control, the development of which 48 hourscorresponded; including 5 daily observed decrease in weight 0.88 ± 0.22, against1.05 ± 0.05 and 8 days in a similar way. As a result of experiments on the effect ofthe Anisakis simplex somatic antigen on the development of chick embryosembryotoxic action installed. Thus antigen has the greatest effect on developmentof early embryos and putting it into the yolk sac.
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