Abstract
In this study, the effect of solvent on the hydrodynamic properties of collagen extracted from tail tendons of young rats was researched. Collagen was dissolved in various aqueous carboxylic acid solutions, including acetic acid (AA), acetic acid with the addition of sodium chloride (AA/NaCl), formic acid (FA), lactic acid (LA), citric acid (CA), and also citrate buffer at pH = 3.7 (CB). The properties of collagen solutions at a concentration of 0.45 mg/mL were characterized based on the viscometric method. The reduced viscosity, intrinsic viscosity, and Huggins coefficient of collagen solutions and effect of solvent, temperature, and UV irradiation on these properties were investigated. Collagen solutions in acetic acid, acetic acid/NaCl, and citrate buffer were irradiated with UV light up to 1 h, and the viscosity of collagen solutions was measured. It was found that the organic acids used as solvent affected viscosity behavior, denaturation temperature, and stability of collagen solutions. The lowest values of studied parameters were obtained for the collagen solutions in acetic acid with the addition of sodium chloride. Thus, the effect of various aqueous carboxylic acid solutions on collagen solutions properties and denaturation temperature can also be affected by the sodium chloride addition. The results of this research can be crucial for the preparation of collagen solutions for both cosmetic and biomedical applications.
Highlights
Collagen is a natural macromolecule isolated from natural sources such as mammal tendons and placenta; feet, skin, and sternal cartilage from domestic birds, for instance, chickens, turkeys, and ducks; bovine skin; and the tendons and bones of buffalos, lamb, rabbits, marine species, and others [1,2,3]
The denaturation temperature is the biggest for collagen in acetic acid solution and it changes as Td AA (38 ◦C) > Td CB (35 ◦C) > Td (33 ◦C) acid/0.2 mol/dm3 aqueous NaCl (AA/NaCl)
It was found that the kinds of solvents show a great influence on viscosity behavior and denaturation temperature
Summary
Collagen is a natural macromolecule isolated from natural sources such as mammal tendons and placenta; feet, skin, and sternal cartilage from domestic birds, for instance, chickens, turkeys, and ducks; bovine skin; and the tendons and bones of buffalos, lamb, rabbits, marine species, and others [1,2,3]. Collagen is not a simple protein, and it is not possible to go to the general conclusion regarding solubility and the effect of solvent on the hydrodynamic properties of collagen of all types. The most widely used collagen in cosmetic and biomedical applications is collagen type I This type of collagen is soluble in acidic pH, and its solubility depends on the age of the tissues from which collagen was extracted. The viscometric properties of a collagen solution depend on the acetic acid or other acids’ concentrations [10]. The interactions between collagen molecules and an acidic solvent are mainly by hydrogen bonds. Preparation of 3D sponges by freeze-drying a collagen solution can be influenced by the type of solvent and its interactions with collagens. After freeze-drying a collagen solution, a small amount of solvent can be found in the sponge
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