Abstract
Standard plot of Thiocolchicoside was constructed in distilled water, 0.01M PBS, n-octanol, ethanol, methanol, 0.9% w/v NaCl and mixture of acetonitrile: water (70:30). Peak of stock solution was observed at a particular wavelength (λmax) and various dilutions of that stock solution were prepared and absorbance value of every dilution was observed at that particular wavelength and corresponding to this regression coefficient (r2), slope value (m) of plot and molar extinction coefficient of drug was observed. As stock solution of Thiocolchicoside was prepared in various solvents with different concentration of stock solution at particular λmax value. All the stock solutions of Thiocolchicoside were scanned in UV region i.e. from 200 nm-400 nm.
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