Abstract
We have found that the adsorption immobilization of the serine protease α-chymotrypsin (Alpha-CT) onto bamboo charcoal powder (BCP), which is a kind of biochar, improves the transesterification rate of N-acetyl-L-tyrosine ethyl ester (N-Ac-Tyr-OEt) with n-butanol (BuOH) in 9 organic solvents. Organic solvents strongly affected the catalysis of BCP-adsorbed Alpha-CT. The transesterification rate of BCP-adsorbed Alpha-CT was much superior to that of free Alpha-CT in every organic solvent. Especially, the transesterification rate of BCP-adsorbed Alpha-CT was about 760 times higher than that of free α-CT in n-butyl acetate.
Highlights
An enormous amount of greenhouse gas such as CO2 has recently been emitted from industries, and thereby has caused serious global warming problems (IPCC, 2014; Olivier et al, 2017)
In order to apply biomass materials to enzyme carriers, we have so far examined the adsorption of enzymes onto biochar, which is derived from biomass materials such as forestry residues
Bamboo charcoal has been prepared by pyrolyzing bamboo waste at low temperatures under nitrogen atmosphere to produce functional groups, which were used as a binding site for the adsorption of enzymes
Summary
An enormous amount of greenhouse gas such as CO2 has recently been emitted from industries, and thereby has caused serious global warming problems (IPCC, 2014; Olivier et al, 2017). The application of biomass materials, which are carbon neutral, to energies and functional materials is crucial to reduce greenhouse gas emissions (Ho & Show, 2015; Straathof, 2014). The development in the high value-added application of biomass materials has been desired to provide the multiple effective utilization system of biomass materials. The main required features of enzyme carriers are chemical stability, thermal stability, insolubility under reaction conditions, high affinity to enzymes, biocompatibility, presence of reactive functional groups, availability, price, regeneration, reusability, and so on. When enzymes are immobilized onto carriers through adsorption, the catalytic activity, specificity, and stability of enzymes are influenced by the nature of carriers. In order to apply biomass materials to enzyme carriers, we have so far examined the adsorption of enzymes onto biochar, which is derived from biomass materials such as forestry residues. We have used bovine pancreas α-chymotrypsin (α-CT) as a model protein since it is well investigated regarding its structure, functions, and properties (Kumar & Venkatesu, 2012)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
