Abstract
We have found that the heat stress tolerance of hen egg white lysozyme (HEWL) is markedly enhanced by the adsorption of HEWL on bamboo charcoal powder (BCP), which is a kind of biochar. HEWL was firmly adsorbed on BCP even at high temperatures. The secondary structure of HEWL was altered to some extent by the adsorption of HEWL on BCP. The remaining activity of BCP-adsorbed HEWL exhibited more than 20% after the incubation for 30 min at 900C although that of free one was hardly observed. Moreover, the half-life of BCP-adsorbed HEWL was 13 min at 900C while that of free one was 4 min.
Highlights
The application of biomass materials, which are carbon neutral, to energies and functional materials is crucial to resolve serious global warming problems since conventional industrial processes tend to produce greenhouse gases such as CO2 (Straathof, 2014; IPCC, 2014; Olivier et al, 2017)
We have found that the heat stress tolerance of hen egg white lysozyme (HEWL) is markedly enhanced by the adsorption of HEWL on bamboo charcoal powder (BCP), which is a kind of biochar
HEWL was firmly adsorbed on BCP even at high temperatures
Summary
The application of biomass materials, which are carbon neutral, to energies and functional materials is crucial to resolve serious global warming problems since conventional industrial processes tend to produce greenhouse gases such as CO2 (Straathof, 2014; IPCC, 2014; Olivier et al, 2017). Forestry residues have scarcely been utilized in the industrial field an enormous amount of forestry residues has been discharged in the world. The development in the high value-added application of forestry residues has been desired to provide the multiple effective utilization system of forestry residues. Bioprocesses such as biotransformation, biosensor, biofuel cell, and so on have been widely studied as sustainable processes (Heinzle et al, 2006). Enzymes are generally stable in a cell. They are gradually denatured and inactivated under various physical and chemical stresses such as heat, organic solvents, and so on (Bailey & Ollas, 1986). In order to enhance the stability of enzymes used in vitro and recycle enzymes, enzyme immobilization has been carried out mainly by attaching enzymes to solid carriers (Zdata et al, 2018)
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