Effect of sodium tanshinone II A sulphonate on rats with acute pulmonary edema induced by sea water drowning and the mechanism involved

Abstract

Objective To investigate the effect of sodium tanshinone ⅡA sulphonate (STS) on rats with pulmonary edema induced by sea water drowning (PE-SWD) and the mechanism involved.Methods Forty Sprague-Dawley rats were randomly divided into 4 groups:the normal group,the STS group,the seawater group (the SW group),the seawater + STS group (the SW + STS group),each consisting of 12 animals.The PE-SWD rat model was established with seawater instillation.PaO2 level,pulmonary pathology,lung wet/dry ratio,pulmonary microvascular permeability (PMVP),the activity of Na+-K+-ATPase,as well as protein abundance and mRNA were monitored respectively.Results Seawater aspiration decreased the level of PaO2 and the activity of Na+-K+-ATPase,and at the same time increased lung wet/dry ratio and PMVP level.Two hours after aspiration of seawater,the level of PaO2 for the SW + STS group increased significantly,when compared with that of the SW group.Pulmonary lesion,W/D ratio and PMVP level for the SW + STS group all alleviated,when compared with those of the SW group.Na+-K+-ATPase activity,Na+-K+-ATPase α1 and β1 protein abundance,and Na+-K+-ATPase β1 mRNA all significantly increased,when compared with those of the SW group.However,no significant differences in the Na+-K+-ATPase α1 mRNA level could be found between the SW + STS group and the SW group.Conclusions STS could produce certain effects on pulmonary edema induced by sea water drowning,the mechanism of which might be related with the level and up-regulation of Na+-K+-ATPase. Key words: Sea water; Drowning; Acute pulmonary edema; Sodium tanshinone Ⅱ A sulphonate ; Na+-K+-ATPase