Effect of smoking on resorption of residual alveolar ridges in complete denture wearers

BackgroundOur aim was to investigate oral health in newly diagnosed head and neck squamous cell carcinoma (HNSCC) patients in relation to long-term survival. We assessed whether the level of alveolar bone loss due to periodontitis at diagnosis, measured from orthopantomogram (OPG), and reported dental health-related quality of life (HRQoL) scores obtained at diagnosis contain prognostic information for HNSCC patients.MethodsA total of 79 patients from a consecutive cohort of 106 diagnosed with HNSCC between November 2002 and June 2005 were included. All patients reported dental HRQoL, OPG-determined alveolar bone loss were measured in 79 patients at diagnosis. Reduced alveolar bone loss (≥ 4 mm) from cement-enamel junction on at least two molars or premolars registered both horizontally and vertically served as indicator of periodontal disease.ResultsWith alveolar bone loss, we determined increased mortality by univariate analysis (RR = 2.28, CI: 1.22–4.28, p = 0.01) and a strong trend by multivariate analyses adjusted for standard clinical information (RR = 1.95, CI: 0.98–3.87, p = 0.056). Reported lowered dental HRQoL scores predicted long-term survival in both univariate (RR = 3.58, CI: 1.99–6.45, p < 0.001) and multivariate adjusted for standard clinical information (RR = 2.17, CI: 1.17–4.01, p = 0.014). When analyzed with Cox regression, including alveolar bone loss and dental HRQoL, both factors, adjusted by clinical variables, were significant predictors of long-term survival: dental HRQoL (p = 0.007) and present alveolar bone loss (p = 0.034). Non-HNSCC disease-specific long-term survival predicted was also predicted when alveolar bone loss and dental HRQoL were analyzed simultaneously and adjusted for standard clinical information.ConclusionsThe degree of alveolar bone loss, as determined by OPG, and dental HRQoL both obtained at the time of HNSCC diagnosis, predicted long-term survival. When analyzed simultaneously, both factors remained significant in both univariate and multivariate analyses, adjusted by pertinent clinical variables, highlighting their unique prognostic value.

Automatic methods for alveolar bone loss degree measurement in periodontitis periapical radiographs

INTRODUCTION: The aim of the study was to observe the relationship between alveolar ridge resorption in mandible and masticatory performance based on gender in complete denture wearers. MATERIALS AND METHODS: The subjects included were those wearing complete denture between the ages of 45-69 years-old, treated at the Dental and Oral Hospital of University Sumatera Utara. Alveolar ridge resorption in mandible was analysed using the panoramic radiograph and masticatory performance was evaluated clinically using the colour-changeable chewing gum. RESULT: 40 patients were included in this study. 50% were women in which 15 women (37.5%) had mild alveolar ridge resorption whilst 5 (12.5%) had severe alveolar ridge resorption. For masticatory performance, 14 women (35%) had good masticatory performance and 6 (15%) had poor masticatory performance. For the men; 17 (42.5%) had mild alveolar ridge resorption whilst 3 (7.5%) had severe alveolar ridge resorption. For masticatory performance, 16 men (40%) had a good masticatory performance and 4 (10%) had a poor masticatory performance. There was a significant correlation between alveolar ridge resorption in mandible and masticatory performance amongst women (p=0.014). However, there was no significant correlation found between gender and alveolar ridge resorption in mandible (p=0.695), between gender and masticatory performance (p=0.716), and between alveolar ridge resorption in mandible and masticatory performance amongst men (p=0.088). CONCLUSION: The alveolar ridge resorption in mandible is associated with masticatory performance amongst women.

Firm lateral force is necessary for the thorough removal of calculus during scaling and root planning (SRP) with hand instruments. However, this firm lateral force should be applied to root surfaces without considering the tooth's loss of supporting tissues. The purpose of the present study was two-fold: firstly, to evaluate the initial pain response of periodontally diseased non-molar teeth with two different levels of alveolar bone loss (ABL) to experimental lateral and vertical pull forces; and secondly, to examine the clinical response of these teeth to non-surgical therapy. Twenty patients with chronic periodontitis were specifically selected in two groups according to the level of ABL at non-molar teeth. Group I consisted of 10 patients who have 141 non-molar teeth with a radiographic evidence of 40-65% ABL. Group II consisted of 10 patients who have 132 non-molar teeth with a radiographic evidence of > or =70% ABL. All patients were systemically healthy, free of pain, and reported no current medication usage. Starting from 0 and gradually increasing an experimental lateral force with digital force gauge, and also an experimental vertical pull force with mechanical force gauge were applied to each tooth and measured. As a result of a single experimental force applied to each non-molar tooth, the initial pain response emerged in the patients was determined by means of electronic bell system used by patients themselves. Each patient was treated with SRP using specific hand instruments under local anesthesia. Plaque index (PI), gingival index (GI), probing depth (PD), clinical attachment level (CAL) and periotest values (PV scores) were compared in both groups at initial and at month 3. A mean experimental lateral force of 24.6 N and a mean experimental vertical pull force of 48.3 N caused initial pain response in group I. Initial pain response occurred with a mean experimental lateral force of 5.3 N and a mean experimental vertical pull force of 19.4 N in group II. Only group I showed statistically significant decrease in PI, GI, PD and a significant attachment gain at month 3 (P < 0.05). There was a decrease of 6 PV in group I at month 3 (P < 0.05), whereas an increase of 4 PV was observed in group II (P > 0.05). This study showed that lateral and vertical forces required for effective SRP do not cause any problem in the group with 40-65% ABL. However, they may cause trauma in the group with approximately 70% ABL. Thus, the results suggest that the degree of healing would be different in the group with > or =70% ABL and in the group with 40-65% ABL.

Colchicine is widely used in the treatment of several inflammatory diseases due to its anti-inflammatory effect, but effects on bone metabolism are unclear. The aim of this study was to evaluate the effects of systemically-administered colchicine on healthy periodontium and experimentally-induced periodontitis. In total, 42 male Wistar rats were included in this study. A non-ligated group constituting the negative control group (Control, C, n = 6) and a ligature-only group forming the positive control group (LO, n = 12) were created separately. Twelve rats were treated with 0.4 mg/kg colchicine and another 12 with 1 mg/kg colchicine. In the colchicine-administered groups, right mandibles constituted the ligated groups (1 mgC-L or 0.4 mgC-L) and left mandibles formed the corresponding non-ligated controls (1mgC or 0.4mgC). Silk ligatures were placed at the gingival margin of the lower first molars. The animals were euthanized at different time-points of healing (11 or 30 days). Alveolar bone loss was clinically measured and TRAP+ osteoclasts, osteoblastic activity, and MMP-1 expression were examined histologically. There was no increase in alveolar bone loss with either colchicine dose in healthy periodontium (p > 0.05) and the highest level of alveolar bone loss, TRAP+ osteoclast number, and MMP-1 expression were measured in the LO group (p < 0.05). The 0.4 mgC-L group showed less alveolar bone loss at 11 days (p < 0.05), but greater loss at 30 days. The 1 mgC-L group showed higher osteoblast number than the other ligated groups (p < 0.05) at both time-points. In summary, colchicine did not increase alveolar bone loss in healthy periodontium and also may tend to reduce periodontitis progression. However, further extensive study is necessary to understand the mechanism of colchicine action on alveolar bone loss in periodontitis.

Radiographic assessment of the relationship between alveolar bone loss and overhanging margins of posterior dental restorations in diabetic patients: a retrospective study.

The plasminogen activating system is a protease/inhibitor system central to extracellular matrix remodeling with a suggested role in periodontal disease pathology. A few studies have reported polymorphisms in the genes of plasminogen activator inhibitors to be associated with periodontal disease severity. Two gene polymorphisms - a BamHI restriction fragment length polymorphism in the urokinase plasminogen activator gene (uPA) and a HindIII restriction fragment length polymorphism in the plasminogen activator inhibitor type 1 gene (PAI-1) - have been associated with conditions having a vascular component, and our objective was to assess the association of these gene polymorphisms with alveolar bone loss in chronic periodontal disease of adults. Genotype was determined by polymerase chain reaction amplification of whole blood, pertinent histories were obtained by interview, and alveolar bone loss was assessed from current radiographs. In 77 elderly patients with a normal distribution of alveolar bone loss, we demonstrated a significant association between levels of alveolar bone loss and these polymorphisms in the uPA and PAI-1 genes. Controlling for the contributions of smoking or diabetes to periodontal bone loss, estimated odds ratios for predicting lower levels of alveolar bone loss, associated with a greater degree of periodontal health, were strongest when defined by the concurrent presence of a homozygous urokinase plasminogen activator genotype and the nuclease-sensitive plasminogen activator inhibitor type 1 (HindIII) allele (odds ratio = 2.6; 95% confidence interval: 5.8-1.3). The urokinase plasminogen activator (BamHI) and plasminogen activator inhibitor type 1 (HindIII) genotypes may serve as useful markers for heritability of bone loss associated with periodontal disease.

Macrophage colony-stimulating factor (M-CSF) and interleukin-34 (IL-34) are ligands for the colony-stimulating factor-1 receptor (CSF-1r) expressed on the surface of monocyte/macrophage lineage cells. The importance of coordinated signaling between M-CSF/receptor activator of the nuclear factor kappa-Β ligand (RANKL) in physiological and pathological bone remodeling and alveolar bone loss in response to oral bacterial colonization is well established. However, our knowledge about the IL-34/RANKL signaling in periodontal bone loss remains limited. Recently published cohort studies have demonstrated that the expression patterns of IL-34 are dramatically elevated in gingival crevicular fluid collected from patients with periodontitis. Therefore, the present study aims to evaluate the effects of IL-34 on osteoclastogenesis in vitro and in experimental ligature-mediated model of periodontitis using male mice. Our initial in vitro study demonstrated increased RANKL-induced osteoclastogenesis of IL-34-primed osteoclast precursors (OCPs) compared to M-CSF-primed OCPs. Using an experimental model of ligature-mediated periodontitis, we further demonstrated elevated expression of IL-34 in periodontal lesions. In contrast, M-CSF levels were dramatically reduced in these periodontal lesions. Furthermore, local injections of mouse recombinant IL-34 protein significantly elevated cathepsin K activity, increased the number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts and promoted alveolar bone loss in periodontitis lesions. In contrast, anti-IL-34 neutralizing monoclonal antibody significantly reduced the level of alveolar bone loss and the number of TRAP-positive osteoclasts in periodontitis lesions. No beneficial effects of locally injected anti-M-CSF neutralizing antibody were observed in periodontal lesions. This study illustrates the role of IL-34 in promoting alveolar bone loss in periodontal lesions and proposes the potential of anti-IL34 monoclonal antibody (mAb)-based therapeutic regimens to suppress alveolar bone loss in periodontitis lesions.

The purpose of the present study was to assess the associations between the presence and amounts of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, B. intermedius, Eikenella corrodens, Wolinella recta, and Fusobacterium nucleatum in the periodontal pocket and the degree of alveolar bone loss and other clinical signs of periodonitis, such as probing pocket depth, attachment level, and presence of bleeding on probing at the same site. The study material comprised 16 subjects with or without approximal sites showing longitudinal alveolar bone loss who were selected from a group of 142 subjects monitored radiographically over the past 4 years. In this group 105 sites were examined, of which 58 showed recent alveolar bone loss greater than or equal to 1 mm. Subgingival plaque was collected with absorbent paper points and hybridized with 32P-labeled DNA probes specific for the above-mentioned bacteria. The amount of each bacterial species was correlated with the degree of bone loss over time and the three clinical measurements by means of Spearman rank correlation. A. actinomycetemcomitans showed poor correlations with all three clinical signs of periodontal inflammation, whereas B. gingivalis and W. recta demonstrated significant positive correlations with the three clinical measurements and with attachment level and pocket depth, respectively. In addition, the amount of A. actinomycetemcomitans, B. gingivalis and W. recta showed significant positive correlation with the extent of alveolar bone loss at the site. In contrast, the amounts of B. intermedius, E. corrodens, and F. nucleatum showed negative correlations with all four measurements.(ABSTRACT TRUNCATED AT 250 WORDS)

Purpose: The purpose of this study was to evaluate the compliance to maintenance schedules recommended supportive periodontal therapy(SPT) and to determine differences in the characteristics of compliant and non-compliant patients. Methods: 414 patients commencing SPT after active periodontal treatment from 2003 to 2005 were included in this study. Based on their compliance with the suggested maintenance schedule, patients were classified as compliant and non-compliant groups. Also patients classified by gender, age, degree of alveolar bone loss and treatment rendered. The association between compliance and patient characteristics was assessed by odds ratio in logistic regression analyses. Results: Only 47% of the initial patient was found to be compliant at the end of August 2008 and 20.8% patients were lost in the first year of SPT. There were significant differences between compliant and non-compliant regard to age, degree of alveolar bone loss and treatment rendered. Conclusions: In conclusion, compliance with SPT generally poor and patients who were older, treated surgical therapy and with mild alveolar bone loss are more compliant to SPT. (J Korean Acad Periodontol 2009;39:193-198)

614 skulls were examined, comprising 49 australoids, 80 negroids, 66 mongoloids and 419 caucasoids. The degree of alveolar bone loss was significantly greater in negroids and australoids than caucasoids or mongoloids. Also the degree of alveolar bone loss decreased between Romano‐British and 19th. century times in caucasoids. A similar pattern of variation was noted in the degree of tooth attrition. The results from this study are not sufficient, however, to assess whether the variation between the various population samples reflect genetic or environmental factors.

summary It has been accepted that the shape of the mandibular base, and especially the gonial angle of the mandible, correlates with the function of the jaw closing muscles. The gonial angles of the mandible and condylar and ramus heights of 30 complete denture wearers (18 women, 12 men, mean age 61 years, range 42–74 years) coming for renewal of their dentures were measured using panoramic radiographs. The mean period of edentulousness was 26 years (range 10–53 years). No statistically significant difference was observed between the sexes in the the sizes of gonial angles and condylar and ramus heights. The right gonial angle was statistically significantly smaller than the left one and correlated negatively with the ramus height in both sides but positively with the increased EMG activity in the right masseter muscle. The size of the gonial angle and the condylar and ramus heights did not correlate with the age of the patients, edentulous period or alveolar ridge resorption.

It has been accepted that the shape of the mandibular base, and especially the gonial angle of the mandible, correlates with the function of the jaw closing muscles. The gonial angles of the mandible and condylar and ramus heights of 30 complete denture wearers (18 women, 12 men, mean age 61 years, range 42-74 years) coming for renewal of their dentures were measured using panoramic radiographs. The mean period of edentulousness was 26 years (range 10-53 years). No statistically significant difference was observed between the sexes in the the sizes of gonial angles and condylar and ramus heights. The right gonial angle was statistically significantly smaller than the left one and correlated negatively with the ramus height in both sides but positively with the increased EMG activity in the right masseter muscle. The size of the gonial angle and the condylar and ramus heights did not correlate with the age of the patients, edentulous period or alveolar ridge resorption.

We aimed to evaluate whether Colocasia antiquorum var. esculenta (CA) mixed with experimental varnish inhibits inflammation and alveolar bone loss in a rat ligature-induced periodontitis model. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) were tested and cell viability of CA were also evaluated. The varnish mixed with CA (CAV) was applied to ligature site of Sprague-Dawley rats and alveolar bone loss and cytokines were measured. CA exhibited a MIC of 31.3 μg/mL and an MBC of 62.5 μg/mL against Porphyromonas gingivalis, with no cytotoxicity. The CAV group exhibited significantly lower levels of alveolar bone loss than the PC group (p<0.05). The expression of IL-1β, TNF-α, and IL-6 was significantly decreased, while that of Runx2 was significantly increased in the CAV than in the PC group (p<0.05). In conclusion, CAV demonstrated the potential to improve the symptoms of periodontitis.

Mandibular bone resorption was studied in patients treated with tissue-integrated prostheses (TIP) and in complete-denture wearers by distance and area measurements on cephalometric radiographs. The area measurements were performed by means of a computer. Two TIP groups with short and long periods (mean = 2.4 and 23.9 years) of edentulousness and wearing complete dentures before fixture installation were followed up over 7-8 years. Mandibular bone resorption was remarkably small behind the distally positioned fixtures in comparison with the corresponding region in complete-denture wearers. Patients with complete dentures showed a continuous resorption of the mandibular alveolar ridge over 21 years, even though there were great individual variations. The bone loss was most pronounced anteriorly and during the first 2 years after extraction of the residual teeth. It is concluded that treatment with tissue-integrated prostheses seems to reduce bone resorption in the mandible, probably owing to favorable load conditions and adequate stimulation of the bone.