Abstract
AbstractThe effect of sialic acid on the electrophoretic mobility (surface charge) of mouse splenic lymphocytes was investigated. On treatment of whole spleen and T cells with neuraminidase, their electrophoretic mobilities decreased to one‐half of the initial values. Calculation of the surface charge from the electrophoretic mobility of lymphocytes before and after treatment with the enzyme indicated that one‐half of the negative surface charge, which determines the electrophoretic mobility, was due to neuraminidase‐sensitive sialic acid in mouse lymphocytes. However, the calculated decrease in the surface charge did not coincide with the amount of sialic acid released from the cell membrane on the enzyme treatment of lymphocytes.
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