Abstract
This experiment was designed to provide information concerning the ability of cell electrophoresis to detect changes in the surface charge of compatible and incompatible lymphocytes as they undergo recognition phenomena. The experimental plan involved the determination of the electrophoretic mobility (EM) of lymphocytes from histocompatible and histoincompatible animal strains; Lewis, Fischer, Buffalo rats and C 57 10J, C 57 10SN and BALB/c mice. A summary and retrospective comparison of the experimental EM data vs. genotypic determinants indicates that: 1. there is no significant difference in the EM's of lymphocytes from individual animals compared with the EM's of mixed lymphocytes from compatible pairs; 2. if the incompatible samples of rat or mouse lymphocytes are mixed, the mean EM value of the mixture is significantly lower than the mean of the independent samples; 3. treatment of cells with neuraminidase decreases their EM and produces a more significant decrease in the EM of the mixture of incompatible cells, than that in the nontreated lymphocytes; 4. the phenomena are not sialic acid dependent.
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