Effect of Shanxian Granule on IFN -γ, TNF -β and IL-10 in peripheral blood of Lewis lung cancer bearing mice

Abstract

Objective: To observe the effect of Shanxian Granule (SXG) on the proliferation of Lewis lung cancer cells and IFN-γ, TNFβand IL-10 in peripheral blood of Lewis lung cancer bearing mice, and explore the molecular mechanism of SXG in inhibiting tumors and improving the anti-tumor immunity of the body, so as to provide a profound theoretical basis for its clinical application. Methods: Lewis lung cancer cells were implanted into subaxillary skin to establish tumor bearing mice model, the mice were divided into control group, model group, chemotherapy group and SXG group, calculating the inhibition rate by weighing the tumor of Lewis lung cancer bearing mice, the changes of IFN -γ,TNF -β and IL-10 in peripheral blood were detected by ELISA. Results: 1. The inhibition rate of SXG group on Lewis lung cancer was 45.99%, significantly higher than that of chemotherapy group (P < 0.05); 2.IFN -γ and TNF -β in peripheral blood of mice in model group and chemotherapy group were significantly lower than those in control group (P < 0.05), while IL-10 was significantly higher (P < 0.05), and IFN -γ and TNF -β in peripheral blood of mice in SXG group were significantly higher than those in model group and chemotherapy group (P < 0.05), while IL-10 was significantly lower (P < 0.05). There was no significant difference in TNF -β and IL-10 between the two groups (P > 0.05). Conclusion: SXG can obviously inhibit the growth of tumor tissue in Lewis lung cancer bearing mice, increase IFN -γ and TNF -β and decrease IL-10 in peripheral blood, suggesting that the anti-tumor effect of SXG may be related to restoring the homeostasis of immune function of cancer patients, improving the immunity of the body and strengthening the immune surveillance function.