Abstract
The mouse mastitis model was used to examine strains of Mycoplasma bovis. Strains that had been passaged in liquid medium more than 60 times were markedly less virulent than the same or different strains with fewer passages. Whereas the low passage strains produced a systemic response in some mice and severe pathological and histopathological changes in the mammary glands of all, the high passage strains produced only minor histopathological changes.
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