Effect of Selection for Pyrethroid Resistance on Abiotic Stress Tolerance in Aedes aegypti from Merida, Yucatan, Mexico.

Abstract

Simple SummaryAedes aegypti is the principal vector of major human pathogens, including dengue, Zika, chikungunya, and yellow fever viruses. Vector control relies mostly on the use of pyrethroid insecticides that kill mosquitoes by disabling the nervous system through binding to the voltage-gated sodium channel (vgsc). Resistance mechanisms have evolved most commonly as mutations in the vgsc gene or in genes associated with detoxification. These mutations are thought to associate with fitness costs, such that the frequency of resistant genotypes should decrease in the absence of insecticide use, and this assumption is critical to managing resistance through insecticide rotation strategies. While most studies to date have investigated life history parameters such as fecundity, we sought to investigate whether environmental stress resistance traits might also vary with insecticide resistance. We found, contrary to our expectations, that a strain selected for enhanced insecticide resistance had higher thermotolerance than its sister insecticide susceptible counterpart. Overall, our results indicate that abiotic resistance traits can correlate with insecticide resistance in surprising and variable ways, potentially complicating the management of insecticide resistance in the field.The study of fitness costs of insecticide resistance mutations in Aedes aegypti has generally been focused on life history parameters such as fecundity, mortality, and energy reserves. In this study we sought to investigate whether trade-offs might also exist between insecticide resistance and other abiotic stress resistance parameters. We evaluated the effects of the selection for permethrin resistance specifically on larval salinity and thermal tolerance. A population of A. aegypti originally from Southern Mexico was split into two strains, one selected for permethrin resistance and the other not. Larvae were reared at different salinities, and the fourth instar larvae were subjected to acute thermal stress; then, survival to both stresses was compared between strains. Contrary to our predictions, we found that insecticide resistance correlated with significantly enhanced larval thermotolerance. We found no clear difference in salinity tolerance between strains. This result suggests that insecticide resistance does not necessarily carry trade-offs in all traits affecting fitness and that successful insecticide resistance management strategies must account for genetic associations between insecticide resistance and abiotic stress resistance, as well as traditional life history parameters.