Abstract
Glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) was extracted from Pteronarcys californica (Plecoptera) by ammonium sulphate fractionation. The enzyme was treated with three forms of mercury (mercuric chloride, methylmercuric chloride, and phenylmercuric chloride) and their in vitro and in vivo effects on GAPDH were studied. It was found that the orders of toxicity were reversed in the in vivo-in vitro experiments. Electrophoresis was conducted on both treated and untreated enzyme, and showed no differences in mobilities between the two. The enzyme was not found to be inhibited by 0·1 mM iodoacetic acid and required cysteine for activity.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
