Effect of seed storage methods on germination growth of Pericopsis mooniana thw. through in-vitro technique

Abstract

Nedun wood (Pericopsis mooniana) is one of the quality local wood species in the Wallacea region. The existence of kayu kuku in their natural habitat is vulnerable by overexploitation and habitat loss. The International Union for Conservation of Nature (IUCN) has declared kayu kuku as one of the endangered species. Tissue culture is one way to conserve genetic resources on micropropagation. The purpose of this study was to determine the effect of seed storage methods on nedun seed germination using in-vitro propagation. There are three methods of storage, which are stored with pericarp at room temperature (T0), storage with pericarp removal at room temperature (T1), storage with pericarp removal in 100C (T2). The variables observed were germination rate, speed of germination, first day of rooted and sprouted, number of roots, length of root, and shoot. ANOVA analysis showed significantly different at shoot length. The duncant test results showed the highest shoot length at T2 (8.75 cm). The largest germination rate was found at T1with an average of 82% and the highest germination speed was T1 (12.23% NG/etmal).