Abstract
This article provides more experimental details and findings of the study as to how the elasticity of scaffold material modulates the gene expression of annulus fibrosus-derived stem cells (AFSCs) (Zhu et al., 2015 [1]). The detailed synthetic route and characterizations of four kinds of biodegradable poly(ether carbonate urethane)ureas (PECUUs) are described. After AFSCs were cultured on electrospun PECUU fibrous scaffolds, the cell proliferation and gene expression analyses were performed to explore the effect of substrate elasticity on the growth and differentiation characteristics of AFSCs.
Highlights
After annulus fibrosus-derived stem cells (AFSCs) were cultured on electrospun poly(ether carbonate urethane)ureas (PECUUs) fibrous scaffolds, the cell proliferation and gene expression analyses were performed to explore the effect of substrate elasticity on the growth and differentiation characteristics of AFSCs. & 2015 The Authors
The gene expression of major matrix components of AF tissue, including collagen-I, collagen-II and aggrecan, after 2 weeks of culture was examined in AFSCs cultured on electrospun PECUU scaffolds using qRT-PCR analysis
The expression of collagen-I gene in AFSCs appeared to increasing with the increase of PECUU elasticity after being cultured for 2 weeks (Fig. 4)
Summary
The PECUUs were synthesized by a one pot, three-step polymerization process as shown in Scheme 1. The ring-opening polymerization of trimethylcarbonate (TMC) was carried out at 110 °C for 24 h with PEO-PPO-PEO as the initiator and stannous octoate as the catalyst. The resulting polydiols were used as soft segment and reacted with 1,6-diisocyanatohexane (HDI) at 75 °C for 4 h under continuous stirring in 15 wt% toluene. 1 wt% putrescine in DMF was added dropwise as a chain extender to obtain PECUU
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