Influence of the Type of Delivery, Use of Oxytocin, and Maternal Age on POU5F1 Gene Expression in Stem Cells Derived from Wharton’s Jelly within the Umbilical Cord

Paulina Gil-Kulik,Piotr Chomik,Ryszard Maciejewski,Mansur Rahnama,Arkadiusz Krzyżanowski,Janusz Kocki,Anna Kwaśniewska,Elżbieta Radzikowska-Büchner

doi:10.1155/2019/1027106

Paulina Gil-Kulik, Piotr Chomik + Show 6 more

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https://doi.org/10.1155/2019/1027106

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Abstract

The paper presents an evaluation of the POU5F1 gene expression in mesenchymal stem cells derived from Wharton's jelly within the umbilical cord, collected from 36 patients during labor. The study is the first one to show that the expression of POU5F1 in mesenchymal stem cells has been dependent on maternal age, birth order, route of delivery, and use of oxytocin. Our research proves that the POU5F1 gene expression in mesenchymal stem cells decreases with each subsequent pregnancy and delivery. Wharton's jelly stem cells obtained from younger women and during their first delivery, as well as patients treated with oxytocin, show higher POU5F1 gene expression when compared with the subsequent deliveries. This leads to a conclusion that they are characterized by a lower level of differentiation, which in turn results in their greater plasticity and greater proliferative potential. Probably, they are also clinically more useful.

Highlights

IntroductionEarly embryonic cells progressively differentiate from the pluripotent state to separate cell lines
During embryogenesis in mammals, early embryonic cells progressively differentiate from the pluripotent state to separate cell lines
Our own studies presented in this study showed that in the group of patients receiving oxytocin, the POU5F1 gene expression in umbilical cord stem cells is statistically significantly higher compared to the expression in stem cells from women in whom oxytocin was not used

Summary

Introduction

Early embryonic cells progressively differentiate from the pluripotent state to separate cell lines. The pluripotency state is transient in vivo; embryonic stem cells (ESCs) derived from the ICM of a blastocyst may possibly retain pluripotency indefinitely in vitro. The POU5F1 (POU domain, class 5, transcription factor 1) gene is a key component of the pluripotency regulation network, and its interaction has proven to be a determinant of the self-renewal of ESCs or their differentiation into trophoblasts [1]. The POU5F1 gene is of key importance for the mechanisms governing pluripotency, and it is strongly expressed in pluripotent cells and is silenced after differentiation. As demonstrated by Guilai and Ying [2] and Niwa et al [3], embryonic stem cells are dependent on the exact level of POU5F1 gene expression [2, 3]

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